Multi-electrode and Imaging Analysis of Xtrypereon Subcellular Connectivity and
Morphology
Chapter 2 -- Preparation
The doctor led Trinity to his exam room and asked her to take a seat on the exam
table that was a hybrid of a dental chair and gynecological table complete with
heavy duty knee stirrups.
"Fancy rig you have there. My doctor just has those little things that look
like pedals to put your feet on when she pokes and prods my cunt." Trinity
commented, running her hand over the stirrups and testing their strength.
"Should I get naked?" she asked, snapping another bubble with her gum as she
perched next to the arm of the exam table.
"Not just yet, unless you would feel more comfortable." he replied, moving to
his desk and picking up several forms which he then attached to his clipboard.
"What I would like to do is get some background on you, then give you a basic
work-up for our needs. I have all your medical records already, but they leave
out information that we need." he stated, looking up at her now.
"How so Doc?" she asked.
He smiled, "I need detailed information about your past sexual activity and I
need to check you for various common conditions that should be done regularly.
Some of your records are out of date. In addition, I need to be sure that you
are not sensitive to some of the controls and dyes we will be using once the
experiment actually begins."
"Cool." she said, shucking her shorts and letting them drop to the floor. "I
love talking about sex, I'm no namby pamby prude like most giggling simpering
females, and I kind of like going to the doctor." She spread her legs and
grinned, "Especially for a pelvic. It turns me on when people look at me and
touch me. Are you going to do a pelvic now?"
He chuckled, "In due time. I take it that you're sexually active?" he asked.
"Who isn't? I've done it dozens of times." she boasted, winking slyly at him.
"Ah, that is good to know as I will have to test you for pregnancy and venereal
diseases." he said, making a notation on his clipboard.
"So then, would you say you are healthy?"
"Oh sure. Healthy enough. I don't get sick much." Trinity replied, snapping
her gum as she spoke.
"Any infections with all the body art?" he continued.
"Nah, not really. A little redness that went away in a couple of days. I heal
fast."
"Any allergies since last time you were checked?"
"Nope."
"Any medical conditions that may not have been listed on your records?"
"Huh? Oh, you mean like heart murmurs and stuff? Nah, not me. Some operation
when I was born or something, but that should be in the records, right?"
The doctor checked, flipping through several pages on the clipboard. "Yes,
right here." "Are you menstruating regularly?" he continued.
"My periods? Yeah, they are pretty regular now."
"Any cramping? Breast tenderness or other pains during your period?"
"Sure, not much though. Want to feel my breasts?" she asked, thrusting out her
chest.
"Shortly. I have a few more questions." he replied.
"How many sexual partners have you had?"
"Ummmm ... let's see .. " she mused, ticking them off on her fingers. "I had
my social studies teacher, he was pretty good but then my folks decided to home
school and so I didn't get to see him anymore. Then there was the man that came
to fix the furnace at home .. he wasn't that good, only wanted me to suck his
cock. Does that count?" she asked.
"Yes, that counts." he replied, making more notations on his pad.
"The best one though was the pastor at my friends church." she grinned. "He
was really hot and taught me a lot!" she tallied up the number, looking
disappointed. "Shit, only three."
"When did you first have sex with a man?" he asked.
"Hmmm ... about 3 years ago I guess. The Pastor dude, you know. My folks
didn't know I was going there during the week, they aren't into church and hell,
neither am I, but he was hot. A couple of times me and Mary did him together."
she giggled slyly. "He loved that and so did I though Mary was terrified she
was going to hell for doing it!"
"So you began when you were 12?" he asked.
"Yeah, nearly 13 I think. I'm advanced for my age." Trinity grinned.
"Have you had sex with a woman?"
"Sure, well, not a grown up woman, just girls my age or a little older. You
know, lots of touchy feely stuff, sucking each other's cunts and putting
different things inside our holes to see how it felt. Nothing as good as a hard
cock though." she added, moving her hand down between her legs to fondle
herself as she spoke.
"Do you masturbate regularly?" he asked, watching her antics with amusement.
"Of course! All the time. See?" she said, spreading her labia with her
fingers and then inserting two fingers inside, pumping them in and out.
"Yes, I see." he replied, again writing on his forms.
"Why all those questions Doc? I thought this was supposed to be a brain study,
not a sex study." she asked, her voice a mix of sarcasm and curiosity.
"The brain is actually the most effective sexual organ we have, believe it or
not. However, this is a brain study and many of the brain's functions are
closely tied to our sexual urges, especially in teenagers. It's an important
aspect we can't overlook." he replied.
Trinity nodded. "Yeah, sure, I get it."
"Would you say you have a happy family life?" he asked.
Trinity scowled. "Happy? Give me a fucking break. My family is so screwed up
I can 't stand it. It's how I got here."
"How so?" he asked with a puzzled frown.
"Well, they don't like me anymore. They like my sisters better. Ever since I
got tits and hair started growing everywhere it's like they've been afraid of
me."
"I'm not sure I understand. You say that now that you are maturing they don't
pay you as much attention?" he asked.
"Yes .. no ... not exactly." Trinity replied, suddenly unwilling to explain her
feelings on the matter.
"It's just that it isn't the same. I know I'm growing up and all, and that I
should be a 'young lady' .. " she said, stressing the word, "but it's like
they're afraid of me or something and it pisses me off."
"I suppose we can explore that later on. That feeling is one that some of our
volunteers have expressed." he noted, rising now.
"Okay then, lie down please." he said, helping her legs into the stirrups and
securing them there with thick leather straps. He moved her arms so that they
rested on the arms of the table and quickly placed straps over her wrists to
secure them there.
Her eyes widened at being in such a vulnerable position. "Isn't this a bit
overkill? This is like from some kinky books I've seen where people get all
tied up and then are helpless while some perverts do disgusting things to them."
He noted the look, "Weren't you looking forward to this part of it?" he asked
with a smile.
Trinity frowned, "This part of what? I was looking forward to a pelvic, not
being some ... some ... well, whatever the hell it's called." she grumbled,
looking nervous now. "Some of the things I read about didn't sound like fun at
all and I'm no sex slave or anything thing like that. When I have sex, I'm in
charge!" she insisted with a scowl.
"It's a regulation of the grant actually, too many volunteers were falling off
and hurting themselves apparently." he replied dryly.
Trinity scowled, "Falling off and hurting themselves? Give me a break, that's a
load of bullshit."
"I completely agree, but I am bound by the protocols in the grant regardless."
he said smoothly. "Besides, it isn't as if you have much choice. However, I am
not a pervert and you won't be subjected to kinky play. I'm just a researcher
working on a very exciting project and sometimes, there just aren't other ways
to get things done that need to get done. Believe me, I am bound by more rules
and protocols than you can think of when it comes to working with human
subjects. The entire experiment will be fully recorded and documented, analyzed
by other scientists, sent for review during the process, and inspectors will be
checking to be sure there are no improprieties and so on down the line. I won't
be able to sneeze without some government official commenting that I didn't
follow proper protocol." he assured her.
Trinity sighed uncertainly then giggled, "Can I report you if you don't sneeze
right?"
He chuckled. "You can report me anytime you feel like you aren't being treated
correctly. I think they will have someone coming in weekly to ask you if
everything is okay and such. If there's a real problem though, I hope you'll
let me know before you take it up the chain of command. Dr. Wingate has warned
me that often I overlook the human aspect of doing all this. I get carried away
by my work and sometimes forget that people need either feedback, or some other
kind of attention from me. I am in some ways very much the absent minded
professor type, disregarding the wants and needs of my volunteers because I get
so wrapped up in what I'm researching."
Trinity didn't reply right away, then asked, "Recorded? Like video and audio
and all that? People will be seeing me, like this maybe?" she said slowly,
apparently relishing the idea though a bit awed and intimidated by it.
"Yes, of course. As I said, with human subjects in experiments, everything is
tightly controlled." He replied absently.
He moved to her head, asking her to open her mouth wide. He plucked the pink
wad of chewing gum from her mouth and tossed it into the trash.
"I'll put this dental clamp in so that I can take swab samples of the mucous and
examine the piercing more closely for signs of damage or infection." he
explained, inserting a metal clamp into her mouth and forcing it open to it's
widest position. She grunted as he did so, her jaw aching from being spread so
widely open.
He snapped on a pair of gloves and began examining her mouth. He took several
swabs worth of mucus samples and then examined the barbell that pierced her
tongue.
"Nice work, clean piercing. Shouldn't give you any trouble." he said.
Trinity rolled her eyes and grunted again, unable to speak properly with the
metal clamp holding her mouth open so widely.
"Now I have to take samples from the contents of your stomach. This will be
rather uncomfortable as I must insert a tube down your esophagus but it should
pass and you'll be fine." he assured her, snapping open a sterile package and
removing a long clear hose with a special plastic plate at one end that was
shaped to fit the mouth.
Trinity's eyes widened and she began to shake her head back and forth, pulling
at her restraints suddenly.
"Now, now, none of that. This won't hurt, it will just be uncomfortable for a
brief period."
He tilted her head back so that it tube would be at the proper angle and guided
the hose expertly downwards. She choked and gagged a bit, but soon overcame it
as the tube was fully inserted. "Probably should have had an assistant do that,
but we weren't expecting to be able to process you today." he commented.
He carefully vacuumed a bit of the contents of her stomach and sealed it in a
vial to be sent to the lab. He then added a funnel to the plate that rested on
her widely stretched mouth and began to pour a liquid into it.
"This is a special compound that will help you evacuate all the material in your
body and relax you a bit for the balance of the procedures." he explained.
She renewed her struggles but was restrained by his hands, and then more thick
leather straps.
"You mustn't struggle so. See why we need all these straps?" he admonished
absently, assembling more medical gear for the balance of the examination.
He removed his gloves and snapped on a fresh pair, stepping between her widely
spread legs now.
He noted that a soft blush came to her face as he did so and that her entire
body tensed.
He added some lubricant to the tips of his fingers and began to work his fingers
into her anal opening, slowly stretching her until she was relaxed enough to
allow a thick tube to be inserted securely. The opaque tube led to a machine
that he now turned on. It hummed softly and Trinity began to groan deeply, her
face deeply red now.
"This tube will remove all the waste from your bowels while I attend to other
matters." he said with an absented-minded smile. "It's good you aren't so
squeamish, that is a relief from other subjects I've had." he noted, apparently
missing her signs of embarrassment.
He snapped off the gloves again and put on a fresh pair. He moved to spread her
labia apart and took a good look at her vaginal area.
"Now for that pelvic you were so eager to have."
Trinity again rolled her eyes
He slipped a finger inside, noting that she was indeed no longer a virgin but
was quite lubricated already. He smiled inwardly.
He chose a very large speculum and took his time working the oversized
instrument into her rather petite vaginal opening. He then slowly opened it,
watching her groan and writhe as the metal forced her tender flesh wide apart so
that he could see inside.
He got a swab and pressed it against her tightly closed cervix, knowing that it
would induce cramping but being quite necessary according to the protocol. He
watched as her muscles flexed and more groans sounded from her throat despite
the tube implanted there.
"A routine and required pap smear as you haven't had one yet though you have
been sexually active." he said, swabbing the plates that would be sent to their
lab for analysis.
"Almost done for now. The rest of the exam will have to take place at Yawla."
he commented, checking her inner labia, urethral opening, clitoris, and prepuce
for structure and function.
He then inserted a catheter into her urethra and drained her bladder. "For the
various tests we have to run, including your pregnancy test."
He then removed the catheter and speculum, patting her vulva as he rose. He
snapped on another pair of gloves and moved a tray of vials and syringes closer.
"Now I just need to fill a few vials with blood so the rest of the tests can be
run." He moved to her arm and quickly wrapped the thin rubber tubing around her
upper arm and then tapped to find a suitable vein. "Good veins. That will make
things easier. Clench your fist a bit ... there ... " he said as he gently
inserted a catheter to allow him to fill several vials for processing. He
filled 5 small vials and then released both the rubber strip and the leather
restraint so that he could apply pressure to the site before covering it with a
small bandage.
"There we go, all set." he said, snapping off his gloves.
"You saved me some time by being clean shaven too. Excellent." he added.
He moved to her head once more, carefully removing the stomach tube but leaving
the dental clamp in place.
"Just a few more minutes and you'll be all done. I'm going to bring these
samples to the lab and then we can get you ready for transport." he said,
rolling the cart with the various samples out of the room, oblivious to her
grunting complaints about the discomfort of her position.
After dropping them off and ordering her prepared for the trip to Yawla, he made
his way to Dr. Wingate's office. He tapped lightly on the door and let himself
in and stopped in surprise to see Dr. Wingate on the sofa apparently nursing
from Trinity's mother.
"I'm sorry to have interrupted." he said with a smile and a nod to Max.
"No problem dear." Dr. Wingate purred, indicating he should take a seat. She
reluctantly let go of Jen's breasts and smiled at her husband.
"Both Max and Jen are ideally suited to be a part of our own experiments and
have graciously agreed to spend their vacation, with their other daughters, at
Yawla." Dr. Wingate informed him.
"Excellent! We've been hoping for just such an opportunity. This will be
wonderful." the doctor exclaimed, obviously pleased at his turn of events.
The doctor smiled at Max, "Was the nursing her idea or yours?" he asked.
Max smiled broadly, "Mine, but I think they were both just waiting to be asked
in any event."
The doctor smiled, "That is true. My dear Wina is quite fond of women,
especially lactating ones. I find it rather fun to watch as well." He added.
Max nodded, "I so enjoy women doing what nature intended them to. How was your
interview with Trinity?"
The doctor chuckled, "I quite understand your frustration at her behavior. She
was quite forward, used horrific language and tried her best to shock me with
sexual innuendo and outright offering herself to me. She is highly sexually
charged and seems to have developed some interesting issues. I am not sure of
their source but she was quite open about her sexual experiences. You knew of
course that she is no longer a virgin?" he asked.
Max and Jen both nodded, sighing. "She made no secret of when she lost it. She
was 12 and decided that she liked seeing a local pastor. We were somewhat
stunned, and yet, she was already developing and had just started menstruating.
Knowing what hormones could be like at that age, we made sure that she
understood about having safe sex and to be sure that she was not being coerced
in any way." Jen explained.
"I was torn between ripping the nuts off the Pastor or just shooting him
outright. However, he actually contacted me about what happened and we had a
very long talk about Trinity. He was actually a decent fellow once I got to
know him. He knew full well I would have killed him had he hurt Trinity. So,
we thought it early, but even back then she had this rebellious streak that
seemed to come from her sexual development. It's only when that streak didn't
settle out over time and in fact got worse that we became deeply concerned, and
then this latest debacle with her friend, well, that was the last straw." Max
added.
The doctor nodded, "Yes, most interesting. I can imagine that she would have
come home, undressed and plopped herself on the kitchen table to show off her
lack of virginity."
Max nodded, "In fact, it happened just about that way. She came home flushed
and excited and so I asked her if she was all right. She was brimming with
excitement, almost hyper and shucked her clothing right there in the living
room, still bloody between the legs and showed me her vagina. I was concerned
that she had been damaged, but she was very proud of what had happened and how
much she had been used. There were still drips of the pastors semen oozing out.
I carefully examined her, cleaned her up and tried to explain the facts of life
once again, but she was totally consumed with her experience and went off to
masturbate before I could make any head way. I recall she was angry at me when
I brushed aside her invitation to sample her myself and in fact, now that I
think of it, she did make some very rude comments hinting that she had done this
because she wasn't getting what she needed at home. At the time, I thought it
was an idle remark, or perhaps something the pastor had said to her, but now I'm
not so sure." Max concluded.
The doctor nodded, raising his brows in surprise. "Most interesting. Losing
her virginity in such a way was perhaps meant to make you feel guilty in some
way, or to draw attention to herself in a most shocking way. I can imagine that
she was quite put out that you took it so calmly though from your own
explanations, she would have had to expect that."
Max nodded. "We've taught our girls to be open and honest, it shouldn't be a
big deal but perhaps that was the wrong thing to do. She's sexually so over the
top and can't seem to control herself. I know she's angry about something, but
I've been unable to figure out what she's acting out against. Did she want us
to punish her for starting so soon? We've never made sexual relations out to be
a sin of any kind ... just the natural course of events as experienced by
humans. Of course, I would have preferred she waited but she was genuinely
thrilled by the experience." Max said slowly, letting the thoughts turn over in
his mind again.
"When you say 'open and honest' what do you mean?" the doctor asked curiously.
"Well, we don't mind nudity around the house of course. We answered all their
questions about their bodies, even after they were developing. They identify
closely with Jen and often compared themselves to her. They walked in on us at
times as we were having sex and we took that rather calmly and in general terms
explained what we were doing. When they asked about various sensations they
experienced on their own, we explained masturbation to them. Just basic stuff,
but Trinity frightens me at times." Max confessed.
Jen nodded, adding softly, "I sometimes wonder if ... well, our lifestyle
contributed to how she is, but I just can't think of how as we have been
extremely careful in what we do and even where and when. I can't help being
what I am ... submissive I mean ... and years ago, I guess it wasn't an issue.
I mean even Lucy Ricardo was spanked by her husband on national television.
Surely whatever we've done couldn't have negatively affected her?" Jen glanced
up, her eyes meeting those of both doctors. "I don't want a justification for
our lifestyle ... but surely .. it didn't cause this?"
"No Jen, I don't think it did in the way you are thinking. If anything, perhaps
the opposite is true, but that will be discovered once we do an in-depth
psychological profile on Trinity." Dr. Wingate assured her.
Max continued, "As you probably noticed, she makes sexual advances on us though
perhaps they aren't sexual in nature. She constantly tries to sit in my lap
and wiggles her bottom, knowing full well the effect it has. She often thrusts
her breasts at me at the same time. She always tries to get Jen to nurse her as
well. That kind of behavior is something I expect from a toddler. She took it
hard when she had to be weaned from Jen's breast and she seems to think we don't
care about her anymore. She's tried to force herself on her sisters, though I
can't really say force as they are at age where they want to explore and who
better with than their older sister who has so much experience? I've made sure
that there was no coercion and have let them explore as much as they wanted.
However, Trinity took that too far with her friend and so she landed in court."
Max explained wearily.
The doctor nodded, "It may be that she is over stimulated, by the brain itself
and so can't properly control her urges. There may also be underlying issues
connected to how she perceives her place in the family. I'm sure we can get it
all sorted out once we begin our experiments." the doctor assured both Max and
Jen.
"If you would finish up my dear, I think we should get going. I've given
Trinity an exam, a good cleaning so that she can travel and the first dose of
the special dyes that will help us with the multi-electrode and image tracking
of the Xtrypereon morphology. I've already had her transferred to the plane
that we will be taking to Yawla." the doctor informed her.
"I don't know when you would be ready to join us? Perhaps tomorrow? I can have
an airplane standing by here so that you could join us. Trinity will be in
surgery the rest of today as we implant the various sensors and devices we need
to track all the neuron activity and so won't really miss you."
Max nodded, "That would be perfect. We have to talk to our daughters, pack, and
close up the house. I have already taken off work as we knew this would be a
difficult time in general with Trinity being away. Hannah and Emma would have
taken that quite hard so I wanted to be there for them. They will be thrilled to
be able to see her, though I will explain about Trinity's surgery and all. They
are sensible girls."
"Excellent. I'll leave contact information for you with my secretary so that
they can escort you to the plane tomorrow. I'll have a nice little apartment
set up so that you'll have a comfortable stay with us in Yawla." the doctor
said, extending his hand to Max.
"It's been a pleasure and welcome aboard." He said with a smile.
"Thank you for the opportunity. Your work sounds very exciting and I am
counting on it helping with Trinity's issues. I'll do whatever I can to help in
the process."
They took their leave of each other -- Max and Jen returning home to their
children to pack, and the doctor and Dr. Wingate to board the plane that would
take them and Trinity to Yawla.
"That went quite well." Dr. Wingate smiled.
"Yes indeed. You think they will be right for our project?" he asked with some
concern.
Dr. Wingate nodded, "Yes, I think they will be splendid actually. Very good
people, open minds, and quite intelligent which is always a bonus. This is a
great beginning to our project dear."
"Yes, I suppose so. They do seem quite reasonable." the doctor agreed. "Well
then, let us attend to our newest charge and see what we can discover."
Trinity was loaded unto a gurney, and carefully restrained with nylon straps for
the flight. An IV was given her to keep her both hydrated and sedated so that
she would be ready for the procedures immediately upon their arrival.
"Shall we begin a tape, or wait until later?" Dr. Wingate asked, checking
Trinity's vital signs carefully and observing the erect state of her nipples and
the lubrication in her vagina.
"I think we will wait. Let's get her stabilized and implanted first. There
will be plenty of time to introduce her to her new world." the doctor said,
also taking careful note of Trinity's state of arousal. One of the drugs in the
IV was designed to create a heightened sensitivity to sexual stimuli so that
those signals would be easiest to record and analyze.
The flight passed uneventfully with both doctors absorbed with studying the
protocols and preparing for the first series of experiments. "Damned
paperwork!" Dr. Wingate cursed, taking the various forms and carefully filling
them out as the plane made its way to Yawla.
Trinity was taken directly to the operating room while both doctors scrubbed up
and organized their team.
The recorders were turned on and the doctor gave an introductory speech to the
team and the various video and audio equipment required by the terms of the
grant.
"As you know, this is our first volunteer for the R01 Multi-electrode and
Imaging Analysis of Xtrypereon Subcellular Connectvity and Morphology grant."
he began.
"In an attempt to create a network of cultured cortical neurons for later use
with a computer simulated body it is necessary to first fully identify and
catalogue the changes in neuronal connectivity and morphology. To build our
database we will implant this subject with a multi-unit electrophysiology and
optical recording with non-destructive 2-photon imaging devices. Changes in
neuronal connectivity and morphology will be followed on the scale of minutes,
hours and days using our 2-photon laser scanning microscope. Two-photon
microscopy allows extended high-resolution imaging of living cells without
harming them or bleaching the fluorescent label."
"The subject will at first be introduced to mundane activities and then more
complex interactions will be introduced and recorded and analyzed. In this in
vitro system capable of behaving and learning, it will be possible to observe
changes in subcellular, cellular, and network properties that underlie learning
and memory. By studying the mechanisms of information processing and storage in
small networks of neurons, models of mental impairment from disease or aging can
be examined with unprecedented detail. Information about how living neural
networks function will promote the creation of more human-like computing
systems."
"Software tools that recognize emergent patterns of network activity will be
developed, and used to trigger distributed patterns of electrical stimulation in
real time. The effects of this sensory-motor feedback loop will be studied at
the millisecond time scale by optical recording using our custom high-speed CCD
camera." he concluded matter of factly.
"The subject will now be prepared for the implantation of the recording
devices." he said, nodding for the nurse to begin shaving Trinity's head while
another went to touch up Trinity's vulva.
In minutes, Trinity was completely bald from head to toe. Her legs were gently
lifted and placed in stirrups and secured. Her arms were stretched out at her
sides and also secured to boards with several nylon straps. Last, her head was
immobilized in a specially designed metal head harness that included a special
dental gag to spread open Trinity's mouth so that a stomach tube could be
introduced. Her eyes were taped shut and the IV reattached.
"We will begin with the 6 cranial sensors and recorders." he said, making a
neat incision on her bald head.
To be continued ...
